Purpose: To assess the prevalence of glaucoma and its types by clinical ophthalmic evaluation of all patients attending the ophthalmology clinic at Sohag University Hospital. Design: A cross sectional observational study. Patients and methods: One-hundred patients underwent full history taking regarding duration of glaucoma diagnosis, treatment regimen and systemic diseases history and full ophthalmic examination including uncorrected and best-corrected visual acuity (UCVA, BCVA), Intraocular pressure(IOP) using Goldmann applanation tonometry (GAT), Automated refraction (AR), Pupil reaction assessment, Angel grading using Van Herick's technique and a standardized ophthalmic examination using slit lamp biomicroscopy for full anterior segment examination, fundus and optic disc examination with 78D aspheric condensing lens. Results: Among 7500 participants, there were 100 patients diagnosed with glaucoma. 51% of cases were females and 49% males and the mean age was 54.3 years (SD ±15.3 years). The crude prevalence of glaucoma was 1.3%. Primary open angle glaucoma was found in 0.76%, primary angle-closure glaucoma in 0.33 %, secondary glaucoma was 0.10% of cases, congenital glaucoma 0.06%, normotensive glaucoma 0.04% and ocular hypertension was 0.02%. Conclusion: Our study found a higher prevalence of glaucoma mainly primary open-angle glaucoma. Age and increased intraocular pressure were significant risk factors of glaucoma. Therefore, increasing public awareness about these risk factors for prevention and early detection of cases is essential and calling for additional observational studies for better understanding of factors.