Purpose: To investigate the effect of refractive errors on anterior chamber parameters in an Egyptian population sample. In addition, we aimed to determine the impact of varying degrees of refractive error on the results of Pentacam and optical biometer measurements of the anterior chamber. Design & Setting: Cross sectional comparative observational study was conducted at Alforsan Eye Center, Assiut, Egypt. Methods: This study included a total of 80 eyes from a sample of 42 Egyptian subjects. The participants aged 18 to 41 years attended the eye centre for routine examinations, prescription glasses, or refractive surgery. Subjects with a history of previous contact lens wearing, ocular trauma, keratoconus, uveitis, or those who had undergone prior ophthalmic or refractive operations were all excluded from the research. The best adjusted and unaided visual acuity, as well as an autorefractometer reading, were obtained. Following a slit lamp examination conducted by the same operators (MS) in a single session, all subjects underwent scanning utilising the NIDEK AL-scan optical biometer and Pentacam oculus (to measure Keratometry (K) measurements, anterior chamber depth (ACD), white to white (WTW) line assessment. Results: Subjects with emmetropia had the shallowest anterior chambers compared to those with myopia. Additionally, the utilization of the Pentacam revealed a statistically significant positive correlation between ACD and keratometry (K2) in the high myopia group. However, a statis-tically significant negative association was detected in the hypermetropia cohort. The use of the AL-scan biometer demonstrated a statistically negative significant correlation between ACD and K2 in low/moderate myopia, hypermetropia, and emmetropia groups. The study revealed that the ACD, K-readings, and WTW measurements obtained from either the Pentacam or the NIDEK AL-scan optical biometer were comparable in all groups. Conclusions: This study demonstrated the existence of a diverse correlation, ranging from positive to negative, between the various anterior chamber parameters and the refractive state of the eye. In addition, the results of the NIDEK AL-scan optical biometer and the Pentacam for ACD and K readings and WTW mea-surements were comparable.