Cotton plants are liable to many pests specially, mites which causing reduction in cotton production. Thus, field experiments were conducted at the Sakha Agriculture Research Station during the seasons 2021 and 2022 to survey and monitor population fluctuations of three mite species and Amblyseius gossypii El-Badry (Barasitiforme: phytoseiidae) on cotton. Also, toxicity of imidacloprid and acetamiprid were examined on the two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae). Twelve species of mites found associated with cotton plants were belonging to 4 families which included mites and its associated predators. T. urticaeand A. gossypii had the highest mite frequency rate. In these studies, the infestation of T. urticae formed two peaks that appeared in mid-Aug. and Sep. in the first and second seasons, respectively. Two peaks of A. gossypii were also appeared in Sep. and Oct. during the two studied seasons. The combined impact of climatic factors on population fluctuation of both T. urticae and Tetranychus cinnabarinus (Boisduval) (Acariformes: Tetranychidae) was insignificant over the two seasons, according to the results. It was highly significant relationships between Tetranychus cucurbitacearum Sayed (Acariformes: Tetranychidae) and RH% during the two seasons and highly significant relationships with A. gossypii during 2022. The combined impact of climatic factors and A. gossypii explained 78.9, 80.1 and 58.5% of the variance (EV) for T. urticae, T. cucurbitacearum, and T. cinnabarinus, respectively, in the first season. In the second season combined impact EV% was 71.5, 82.9 and 61.3 % for the three mites, respectively. Imidacloprid (seed treatment) was the least toxic with total mean reduction percentages of 30.3 and 42.6 % in 2021 and 2022, respectively. While acetamiprid was the most effective on sprayed plants, with a reduction percentage of 96.72 and 97.78% during the first and second seasons, respectively. These data revealed that the correlation between mites, predators, and climatic factors was conflicting, and imidacloprid and acetamiprid can significantly minimize the infestation of T. urticae on cotton plants and be used in IPM programs.