The housefly is considered a major household pest, as it carries many pathogens that threaten human health. The increase in awareness of the dangers of chemical insecticides on the environment and the increase in insect resistance to various pesticides, led to the continuation of the search for safe and alternative sources of insecticides. The present study was carried out using crude rice starch paste as a natural bio reducing agent to produce green synthesis of AgNPs due to its cheapest and safety on environment. The larvicidal effects of (AgNPs) were tested against the third larval stage of both strains (the susceptible laboratory strain). (SS) and field strain (Gol-RR) of the house fly. Larvae were fed on contaminated medium with AgNPs concentrations at 0, 10, 20, 30, 40 and 50 μg /ml. The numbers of dead larvae were recorded in both strains, at intervals 24, 48 and 72 h. Then, larvae were tracked for survival to adulthood and observed different biological aspects. The results showed that the larval mortality rate of both strains of the house fly, increased with increasing concentrations and increased gradually with increasing the exposure period, while it was found that the comparison treatment did not show any mortality. However, silver nanoparticles (AgNPs) at a concentration of 50μg/ml recorded maximum larval death (%) in both strains of Musca domestica, followed by 40 μg /ml. But the susceptible strain was affected more often than the field strain, followed by a significantly higher number of abortive fledgling adults, which show morphological abnormalities.