Virgin coconut oil ( VCO) can be used as a functional food because it provides many health benefits in addition to its nutritients agent. It also contains antioxidants as well as antimicrobial and anticancer activity. The chemical composition of VCO was identified by gas chromatography–mass spectrometry GC-MS analysis. The antioxidants present in VCO were identified and polyphenols as well as flavonoids were identified using (GC-MS) and HPLC analysis. The well diffusion assay method was used to investigate the antimicrobial activity of VCO. The SRB assay assay method was used to investigate the anticancer activity of VCO. . Nine fatty acids were quantitively identified in VCO and it richs in saturated fatty acids, with high Percentge of lauric and myristic acids . GC-MS analysis and HPLC analysis of compounds contained in VCO resulted in of 17 antioxidant compounds .( VCO)has no effect on the microbes used in the sense that it has no microbial effect , VCOhas anti-cancer activity, So, this study concluded that VCO is a functional food with many health benefits in addition to its nutritive value