Background
Fluorescence in situ hybridization (FISH) is highly accurate in detecting specific genetic rearrangements or gene amplifications. It provides direct visualization of chromosomal abnormalities or gene fusions at the cellular level.
Objective
This article investigated the molecular labeling of maxillofacial lesions and examined the detectable genetic alterations using FISH analysis. The study discussed the merits and limits of FISH analysis in diagnosing these lesions and provided insights into the future applicability of FISH as a diagnostic tool.
Method
We conducted a comprehensive search of electronic databases (e.g., PubMed, Embase, Web of Science) to identify relevant studies published within a specific time frame. We utilized appropriate search terms, including "maxillofacial lesions," "fluorescence in situ hybridization," "genetic alterations," "diagnosis," and related keywords .Included articles published in English, targeting human subjects, and focused on the molecular characterization of maxillofacial lesions using FISH analysis. Iterative refinement and validation were used for improving the accuracy of predictions of the timeseries analysis.
Results
We identified the limitations and challenges associated with FISH analysis in maxillofacial lesions, such as the nonspecificity of genetic alterations and the limited availability of fusion probes. We analyzed the implications of these findings and inferred the future applicability of FISH analysis in the diagnosis of maxillofacial lesions.
Advances in Knowledge
The evolving landscape of molecular diagnostic techniques uses more trendy and efficient techniques than FISH analysis. With competing such techniques, FISH is less likely to be of use soon unless promising avenues are to be proposed for overcoming existing limitations.