The purpose of the current study was to determine the toxicity of three compounds to Psammotermes hypostoma Desneux, a subterranean termite (Isoptera: Rhinotermitidae): chlorpyrifos, imidacloprid, and lambada cyanothrin. In the agricultural and rural districts of the Sohag governorate in Egypt, P. hypostoma termites are regarded as some of the deadiest termites. The cardboard dip bioassay method is being used in a lab setting. In comparison to imidacloprid and lambada, the results demonstrated that chlorpyrifos gave control of the termites' population after 3, 12, and 24 hours of application on setts. Following three hours, the highest values for LC50 and LC90 were 37.74 and 837.34 ppm for chlorpyrifos, whereas the lowest values were 166.11 and 7685.44 ppm for lambada. The mortality rose as the duration was extended; after24 hours, the chlorpyrifos LC50 and LC90 were 7.92 and 97.74 ppm, respectively. After 90 days of treatment, subterranean termites, P. hypostoma, dramatically reduced the percentage of infested trees treated with chlorpyrifos and imidacloprid; lambada showed the lowest reduction percentage. Furthermore, imidacloprid's effectiveness and sideeffects continued for up to 105 days following treatment, while chlorpyrifos's lasted for up to 120 days. According to the study's findings, out of all the insecticides tested, chlorpyrifos was the most effective pesticide.