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335645

Suppressing pathogenic fungi associated with stored garlic bulbs causing cloves rot and decreasing disease development during storage by Bacillus subtilis and Trichoderma harzianu

Article

Last updated: 05 Jan 2025

Subjects

-

Tags

Agricultural Biotechnology.

Abstract

This study investigated the pathogenic fungi associated with 
stored garlic bulbs causing cloves rot (CR) disease. First, 25 fungal 
isolates were obtained from naturally diseased samples of stored garlic 
bulbs showing cloves rot symptoms collected from different counties of 
Sohag governorate and identified as Aspergillus niger van Tieghem (4 
isolates), Botrytis allii. (4 isolates), F. oxysporum Schlecht. (5 isolates), 
F. proliferatum (Matsush.) Nirenberg (4 isolates), F. solani (Mart.) 
Sacc. (4 isolates), and Penicillium sp. (4 isolates). Ihe pathogenicity test 
conducted on cloves and seedlings under ambient laboratory and 
greenhouse conditions. All isolates belonging to Fusarium spp. were 
superior to other tested fungal isolates, causing the highest infection of 
CR and recovered from infected tissues of garlic cloves. Also, all 
isolates of F. oxysporum caused seedlings' damping-off and were 
superior to other tested isolates of F. solani. Under the greenhouse 
conditions, a significant decline in cloves germination and increased CR 
values of the disease severity index (DSI) occurred on garlic plants 
inoculated with all isolates of F. proliferatum and F. oxysporum. On the 
other hand, all isolates of F. proliferatum and F. oxysporum exhibited 
high values of the DSI after 60 days of bulb storage at room conditions. 
In vitro tests, all tested bacterial and fungal isolates significantly 
inhibited the mycelial growth of F. oxysporum and F. proliferatum. 
However, isolates of T. harzianum were more effective in reducing the 
mycelial growth of both fungi than isolates of B. subtilis. In the 
greenhouse trial, both tested antagonists, B. subtilis (isolate No. 2) and 
T. harzianum (isolate No. 5), significantly increased cloves germination, 
reduced the DSI of cloves rot caused by both fungi, and decreased 
cloves rot disease development of garlic during storage. Under field 
conditions, both tested antagonists significantly increased cloves 
germination and reduced the DSI of cloves rot caused by both fungi, as 
well as decreased the development of garlic cloves rot disease during 
storage under room conditions

DOI

10.21608/jsasj.2023.335645

Keywords

garlic cloves rot, F. oxysporum, F. proliferatum. Trichoderma sp, B. subtilis. Bological control

Authors

First Name

Mazhar

Last Name

Mohamed

MiddleName

Desouki

Affiliation

Agricultural microbiology Department, Faculty of Agriculture, Sohag university, Sohag, Egypt.

Email

mazhareisawi@agr.sohag.edu.eg

City

Sohag

Orcid

-

First Name

Moustafa

Last Name

Moharam

MiddleName

H.A.

Affiliation

plant pathology dept., faculty of agriculture, sohag univ.

Email

mostafa.moharam@agr.sohag.edu.eg

City

-

Orcid

-

Volume

8

Article Issue

2

Related Issue

45303

Issue Date

2023-12-01

Receive Date

2024-01-10

Publish Date

2023-12-01

Page Start

329

Page End

339

Print ISSN

2357-0725

Online ISSN

2735-5578

Link

https://jsasj.journals.ekb.eg/article_335645.html

Detail API

https://jsasj.journals.ekb.eg/service?article_code=335645

Order

335,645

Type

Research and Review Papers

Type Code

1,734

Publication Type

Journal

Publication Title

Journal of Sohag Agriscience (JSAS)

Publication Link

https://jsasj.journals.ekb.eg/

MainTitle

Suppressing pathogenic fungi associated with stored garlic bulbs causing cloves rot and decreasing disease development during storage by Bacillus subtilis and Trichoderma harzianum

Details

Type

Article

Created At

28 Dec 2024