The development of simple, economical, and 
effective technologies to produce nanoparticles is a global 
priority. Microorganism-based biosynthesis methods 
developed as an environmentally conscious, pristine, and 
feasible alternative to conventional physical and chemical 
approaches. This research outlines the synthesis of copper 
oxide nanoparticles utilizing bacterial filtrated supernatant. 
The investigation focused on variables such as pH, copper 
concentration, and temperature. Optimal conditions for 
nanoparticle formation were identified at a neutral pH, 4 
mM copper concentration, and 35°C. Characterization of 
the CuO NPs involved TEM, UV-Vis spectroscopy, XRD, 
and FTIR to examine their pure form, revealing a maximum 
particle size of 36.22 nm with spherical morphology. 
Evaluating the antimicrobial efficacy on pathogenic bacteria 
(Staphylococcus aureus and Pseudomonas aeruginosa) and 
plant pathogenic fungi (Rhizoctonia solani and 
Fusariumsolani) through the well diffusion method 
affirmed the probable outcome of CuO-NPs as an efficient 
antimicrobial agent, supporting and validating prior 
assertions