The development of innovative, effective, and safe varocides 
based on essential oil nanoemulsions (NEO) will assist to 
mitigate the negative effects of synthetic acaricides. To use 
ultrasonic emulsification to synthesize a nanoemulsion of 
essential oils and test their biological activity against the varroa 
mite and selectivity for Apis mellifera. When bioassayed with 
surface contact toxicity and exposure time of 4 hours, the LC50
values of all investigated nanoemulsions were (1.06, 3.11, 1.66, 
and 1.47 µg/cm2
) of NEO of Garlic, Camphor, Chamomile, and 
Jojoba, respectively, with no significant variations between them. 
The LC50 of NEO oils were (0.610.23, 1.020.22, 0.710.24, and 
0.640.24 µg/cm2
) as exposure time increased.to 24 hours of 
exposure, The data showed that all Nano-emulsion oils (NEO) 
are good selective after 24 hours of exposure. The maximum 
selective ratio was on Chamomile treatment followed by Jojoba 
(452.27 and 427.65%). The average reduction infestation 
percentage of all NEO ranged between 72.56 and 78.96% against 
V. destructor without any significant differences between them. 
Their degree of selectivity was good selective against bee 
workers. All tested nanoemulsion oils have no detrimental effects 
on honey bees and colonies. NEO can be part of an IPM program 
to control varroa mites and is considered an ideal way to control 
this mite. These materials are therefore environmentally safe, 
selective for bees, easy to use, available, and economical.