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334599

Isolation and characterization of actinophages specific to Streptomyces rochi from Sohag Governorate

Article

Last updated: 05 Jan 2025

Subjects

-

Tags

Agricultural Biotechnology.

Abstract

The objective of this study was to identify and separate 
Streptomyces rochei-specific actinophages. From two 
different sites of reclaimed soil in the Sohag Governorate 
region, eight actinophage (streptomyces phage) isolates 
specifically for Streptomyces rochei were successfully 
identified. The samples were taken from reclaimed soil in 
Alkawmel city and the New Sohag experimental farm of the 
Faculty of Agriculture, Sohag University, in Sohag, Egypt. 
All of the isolated phages (the actinophage) looked to be 
head-and-tail types, according to electron micrographs of 
the isolated phages. The eight isolates of the phage were 
given the numbers 1, 2, 3, 4, 5, 6, 7, and 8. Each phage 
isolate's heat inactivation point was investigated. Although 
all phage isolates maintained their infectivity at 85 °C 
during 10 minutes, they all demonstrated resistance to high 
temperatures. It has been discovered that a pH of 7 is ideal 
for phage infection, while phage isolates can survive in both 
alkaline and acidic environments. The susceptibility of the 
different phage isolates to UV light varies. 

DOI

10.21608/jsasj.2023.334599

Keywords

Actinophages, streptomyces rochi, Soahg

Authors

First Name

Mazhar

Last Name

Mohamed

MiddleName

D A

Affiliation

Agricultural Microbiology Department Faculty of Agriculture Sohag University Sohag 82524 Egypt

Email

mazhareisawi@agr.sohag.edu.eg

City

-

Orcid

-

First Name

Christeena

Last Name

Mankarious

MiddleName

O F

Affiliation

Agricultural Microbiology Department Faculty of Agriculture Sohag University Sohag 82524 Egypt

Email

-

City

-

Orcid

-

First Name

Adel

Last Name

Hammad

MiddleName

M M

Affiliation

Agricultural Microbiology Department Faculty of Agriculture Minia University Minia Egypt

Email

amhammad2000@yahoo.com

City

-

Orcid

-

First Name

Tarek

Last Name

Elsharouny

MiddleName

H M

Affiliation

Agricultural Microbiology Department Faculty of Agriculture Sohag University Sohag 82524 Egypt

Email

-

City

-

Orcid

-

Volume

8

Article Issue

1

Related Issue

43354

Issue Date

2023-06-01

Receive Date

2024-01-06

Publish Date

2023-06-01

Page Start

136

Page End

145

Print ISSN

2357-0725

Online ISSN

2735-5578

Link

https://jsasj.journals.ekb.eg/article_334599.html

Detail API

https://jsasj.journals.ekb.eg/service?article_code=334599

Order

334,599

Type

Research and Review Papers

Type Code

1,734

Publication Type

Journal

Publication Title

Journal of Sohag Agriscience (JSAS)

Publication Link

https://jsasj.journals.ekb.eg/

MainTitle

Isolation and characterization of actinophages specific to Streptomyces rochi from Sohag Governorate

Details

Type

Article

Created At

28 Dec 2024