Tomato plants are affected by numerous environmental 
factors, especially biotic stresses such as fungi, bacteria, viruses, 
nematodes, and insect pests while growing that lead to yield 
damage. Globally, Fusarium oxysporum f. sp. lycopersici is 
considered a crucial pathogen that has been documented to cause 
damage to tomatoes by decreasing the yield in both open fields 
and the greenhouse. Fusarium wilt is a soil-borne fungal 
pathogen capable of surviving in the soil and crop debris for a 
decade and has been known to enter the plant through wounds on 
roots. SDS-PAGE analysis was done in eight isolates of
Fusarium oxysporum f.sp. lycopersici. The results revealed that 
the studied isolates of Fusarium were clearly distinguished from 
each other at the studied biochemical level. The dendrogram 
obtained from the data showed that hierarchical clustering 
separated the isolates into three groups according to their 
similarity coefficients, confirming that some protein-based 
markers were associated. Therefore, biochemical markers 
associated with highly pathogenic Fusarium isolates offered a 
promising alternative to morphological markers to decide the 
appropriate selection of high pathogeneses for many biological 
applications.