Ewaise mango is the most delicious mango varieties in Egypt. It is very popular. However, it suffers of low fruit yield. The shortage of yield may be due to the adverse effects of the unfavorable environmental conditions. Amino acids are proved to improve these negative effects. In this study, Ewaise mango trees grown during 2020 and 2021 seasons were treated with four amino acids; aspartic acid 50 ppm, tryptophan 50 ppm, methionine 50 ppm and glycine 50 ppm alone or in combination. Amino acids application improved plant growth and development and fruit quality comparing to control. The combination of the four amino acids yielded the highest values of leaves area, chlorophyll and nutrients content, yield and its components and the physical and chemical traits of the fruit. To enhance yield and fruit quality of Ewaise mango trees, it is suggested to use three sprays of a mixture containing (aspartic acid 50 ppm, tryptophan 50 ppm, methionine 50 ppm and glycine 50 ppm).The objective of this study is to investigate the enhancement of Ewaise mango crop by application of amino acids.