A field experiment was conducted at the Agricultural Research Farm at Al-Kawthar site, Faculty of Agriculture, Sohag University, Egypt, during the 2018/19 season, to evaluate 10 faba bean genotypes as well as their morphological and anatomical root traits to determine the genotype that is the most tolerant of Orobanche infection under naturally infested soil conditions. Randomized complete blocks design (RCBD) in a plots arrangement with three replicates was used. The experiment included 10 faba bean genotypes (Sakha 1, Giza 843, Giza 716, Nubaria 1, Misr 3, Assiut 62, Assiut 85, Assiut 115, Assiut 125 and Assiut 215). The results revealed that the genotypes Giza 843 and Misr 3 were more tolerance to broomrape than other genotypes and recorded the lowest values for the number of broomrape (spikes/m2) and the dry weight of broomrape spikes (g/m2). Furthermore, genotypes Giza 843 and Misr 3 produced the highest biological yield and seed yield (ton/fed) under these conditions. While Nubaria 1 and Giza 716 genotypes were highly susceptible to broomrape infection and recorded the highest values for the number of broomrape (spikes/m2) and the dry weight of broomrape spikes (g/m2), they also gave the lowest values for the biological yield and seed yield (ton/fed) under these conditions. Correlation analysis among the studied traits also showed that the root length was positively, significantly and strongly correlated with biological yield and seed yield (ton/fed). On the other hand, the root length was negatively, highly significantly and strongly correlated with the number of broomrape and the dry weight of broomrape spikes. The fresh weight of root was positively, highly significantly and strongly correlated with the number of broomrape and the dry weight of broomrape spikes. While, the fresh weight of root was negatively, highly significantly and strongly correlated with biological yield and seed yield (ton/fed). These findings suggest that the vascular system of cultivars Giza 843 and Misr 3 root cells played a significant role in the tolerance of broomrape infestation compared to other susceptible genotypes to broomrape infection, such as genotype Giza 716 and the highly susceptible genotype Nubaria 1. Finally, it can be concluded that many defense mechanisms have been detected in faba bean plants that tolerate broomrape attack, mainly involving cell wall reinforcement and stamping of vascular tissues with a decrease in the secretion of germ stimulants.