This experiment was carried out at the Experimental Farm of Shandaweel Agricultural Research Station, Sohag, (ARC), during 2018/2019 and 2019/2020 seasons, to study the effect of nano fertilizers through injection on growth, yield and quality of onion Giza 6 Mohassan cultivar under Sohag conditions. Four different mineral (NPK) treatments (100% NPK, 75% NPK, 50% NPK and 25% NPK) and four different injections with nano NPK [(control (treatment without injection), 5, 10, and 15 L fed.-1 in addation to the control treatment (without injection) were applied. The experiment was conducted in split plot design with three replicates. The different mineral NPK treatments were arranged in the main plot and the different injection with nano NPK treatments were assigned to sub plots. The results showed that both of the mineral NPK rates and the injection onion with nano NPK rates significantly affected most of studied traits, in the both seasons. The interaction between the two studied factors, significantly affected both total and exportable bulb yield traits in two seasons. The injection with the nano NPK rate (10 L fed.-1) and the mineral NPK (75% NPK and 100% NPK) achieved the highest values of total bulb yield (21.99 and 21.85) ton fed.-1. in the first and second season, respectively. While, the treatment without injection (control treatment) with the lowest mineral NPK (25% NPK) gave the lowest values of total bulb yield (14.06 and 12.75) ton fed.- in the first and second season, respectively. The injection with the nano NPK rate (15 L fed.-1 and 10 L fed.-1) achieved the highest values of exportable bulb yield (18.51 and 18.56) ton fed.-1 in the first and second season, respectively. Therefore, the results of this study recommend the use of mineral NPK combined with nano NPK as soil injection (75% mineral NPK + 10L fed.-1 nano NPK), to increase the total and exportale yield of onion, reduce environmental pollution by providing a quarter of the recommended amount of chemical fertilizers and saving hard currency by reducing the import of chemical fertilizers.