The descriptive homozygous selection of resistance genes of the two-spotted spider mites was to five acaricides (malathion, abamectin, fenpyroximate, bromopropylate and bifenthrin) of different chemical classes. Females were treated by the diagnostic concentrations of these acaricides, which obtained crossing with diheterozygous females. Hybrid females showed no resistance to abamectin, fenpyroximate, brompropylate, and bifenthrin, but the mortality under malathion increased 1.5-2 time compared with homozygous females. Epistatic interaction of resistance genes was manifested due to incompatibility of biochemical processes of protection against poisoning determined by them to toxicants of different chemical classes. The coefficient of correlation R2 and the regression coefficient β were subjected to present the dependency of Y (dependent variable) upon X (independent variable). On the other hand, the regression coefficient shows that a unit change in X variable will bring change in Y variable. In addition, Euclidean distance analysis distinguish the superiority resistant genes throughout the chemical classes.