The adverse effects of antibiotics are still a vital problem in human life. Authors in this work have examined the negative impacts of one of the most widely used drugs, amoxicillin (AM), on the testes of male rats, and also addressed how to deteriorate these effects by utilizing a natural product, Ashwagandha seeds extract (ASE) by comparing the role of the extract as a protective and therapeutic agent. Histopathological analysis of the testes showed that ASE could enable the biosystem to defend the testicular tissue against the toxic effects of AM. It proved that the protective use of ASE was better. Moreover, dielectric and thermodynamic parameters (electrical impedance, relaxation time, electrical conductivity, activation energy, and change in enthalpy) revealed their aptitude in discriminating the toxic AM and ameliorative ASE impacts. The medical action of ASE is due to its content of polyphenols and flavonoids. In other words, ASE proved its ability to help the biosystem repair some of the AM side effects on testes' tissues, and dielectric parameters proved their capability to detect toxicity and repair in testes tissues.