Excessive use of antibiotics has side effects on the bio-system. The biochemical reasons for such effects are not always known. In this study, the impact of Amoxicillin, AM, on the liver tissue was investigated by a physical parameter, admittance spectroscopy. Also, the effect of a natural supplement, Ashwagandha seeds extract (ASE) (100, 200, 300 mg/kg) as a protective agent taken with AM and as a therapeutic agent given after ceasing AM were measured by comparing their effects on the liver admittance. Results showed a reduction in liver admittance and a reverse of the electrical behavior of liver as a result of AM toxicity. On the other side, the protective and therapeutic ASE reduced AM–induced liver toxicity. By comparing the role of protective and therapeutic ASE, the therapeutic doses could restore the admittance values nearer to control. These findings proved that electrical admittance can be used as a sign of liver tissue status.