ABSTRACT The objective of the research was to evaluate the potential safeguarding impact of basil seeds and ther oil on kidney damage caused by zinc oxide nanoparticles (ZnONPs) in male albino rats.  Four groups of twenty-four male Sprague -Dawley rats each were formed. The rats in the first group (the control group) were fed a baseline diet. At the end of the trial involved administering 600 mg/kg of ZnO nanoparticles intravenously to the second, third, and fourth groups of rats, along feeding with a basic diet, 5% basil seed, and 5% basil oil, respectively. The experiment involved drawing blood samples, gathering internal organs, and weighing them at the end. A complete blood count was performed. The serum was separated for biochemical examination. Serum creatinine, uric acid, urea, zinc, calcium, magnesium, sodium, potassium, and the renal levels of malondialdehyde increased after injection of ZnONPs, as did reduced hemoglobin, creatinine clearance, zinc's renal activity, and antioxidant enzymes. On the other hand, the administration of basil seeds and their oil improved kidney functions and renal antioxidant enzymes. Therefore, basil seeds and their oil can potentially be preventive against ZnONPs-induced nephrotoxicity.