Mangosteen (Garcinia mangostana L.) is famous as the queen of fruits and considered as natural health-promoting dietary supplement. Recently, mangosteen fruit has received a great deal of attention due to the therapeutic properties in treating different diseases throughout the world. However, scientific studies in Egypt on the effects of mangosteen in vivo are very rare. This work aims to assess the protective effect of mangosteen fruit extract with three doses (1, 2 and 3ml/kg b.w) in benign prostatic hyperplasia induced by testosterone in male rats. Thirty-six rats weighing between 190-200g, divided into six groups (6 rats for each); one served as normal control group (N.C) (-ve) , BPH (+ve), BPH+ zinc (20 mg/kg b.w), BPH + MFE1 (1ml/kg b.w), BPH + MFE2 (2ml/kg b.w) and BPH + MFE3 (3ml/kg b.w), in order to induce BPH, rats were injected by testosterone (5 mg/kg b.w) daily. At the 29 day, all rats were sacrificed and their serum and prostate were analyzed. The results showed that mangosteen with different doses (1,2 and 3ml/kg bw) significantly diminish the development of benign prostatic hyperplasia by decreasing (prostate volume, prostate weight, prostate weight index and testosterone levels) and increased the levels of LH, FSH and total protein in serum in all protective groups which received different doses of MFE in comparison with BPH (+ve) untreated. In conclusion mangosteen fruit extract with different levels has some significant protective effects on reproductive functions in male rats.