ABSTRACT This study aimed to investigate the protective effect of Bidens Pilosa (B.Pilosa) leaves against carbon tetrachloride (CCl4)-induced hepatotoxicity in rats and to determine its phytochemical constituents. Forty eight adult males albino rats were distributed into six groups (n=8). Group (1) fed on basal diet B.D and used as a control negative group, (2) fed on B.D and injected with carbon tetrachloride CCl4 (2mlL/kg) twice a week for two consecutive weeks to induce hepatotoxicity in rats and used as control positive group, Groups (3), (4), (5) and (6) injected groups were fed on B.D and treated daily with 75, 150, 300 and 450mg/kg of B.pilosa extract, respectively. At the end of experiment blood samples were collected from each rat on fasting state for biochemical analysis, the blood samples were examined for  liver enzymes activities (ALT and AST), lipid profile including total cholesterol, HDL, LDL and VLDL, and kidney function including ( uric acid & urea nitrogen). The result showed that dried powder of B.pilosa leaves contains carbohydrates, glycosides, sterols, triterpenoids, flavonoids, tannins, saponins and alkaloids. B.pilosa extract increased body weight gain, liver enzyme ALT,  AST, LDL,VLDL , uric acid and urea nitrogen on the other hand HDL was increased. Therefore B.pilosa may be beneficial for patient suffering from liver toxicity.