The study evaluated the efficacy of Aspergillus niger and Fusarium proliferatum as mat and pellet biomasses for wastewater treatment as a mycoremediation method. In synthetic wastewater, A. niger mat form demonstrated a maximum removal efficiency of (80.20^a ± 0.16%) for organic pollutants under specific conditions (pH 6, 0.1 gm in 100 ml, 7 days at 150 rpm, 30°C). However, mycelial pellets of this fungus achieved a higher maximum removal efficiency of (86.96^a ±0.05%) for organic pollutants (at a concentration of 65.20 ±0.23 mg/L) under different conditions (pH 7, 0.2 gm in 100 ml, 5 days at 150 rpm, 30°C). F. proliferatum's mycelial mat demonstrated a maximum removal efficiency of 48.07a ±0.08% for organic pollutants under specific conditions at a concentration of 259.65 ± 0.42 mg/L, pH 6, 0.2 gm in 100 ml, 7 days at 150 rpm, 30°C), Mycelial pellets demonstrated maximum removal efficiency of 33.57a ± 0.11% for organic pollutants under various conditions (pH 6, 0.2 gm in 100 ml, 7 days at 150 rpm, 30°C). The study found that A. niger pellet had significantly higher removal efficiency than mat form, while F. proliferatum mat and pellet forms showed similar differences but lower values. A. niger is generally more effective in decomposing water pollutants, offering scalability in bioremediation applications, ease of production in labs, and pH tolerance. The study suggests that fungal biomass, particularly pellets, can effectively and eco-friendly treat wastewater with variable acidic or alkaline conditions through mycoremediation.