Ehrlich ascites carcinoma (EAC) are undifferentiated, natural mouse mammary adenocarcinomas that are primarily used for research. The current study aims to assess whether loaded ginger nanoparticles have a protective effect against renal toxicity induced by EAC in mice. This study employed forty-nine mice, which were separated into seven groups as follows: Group1(GR1) was utilized as the negative control. EACs were implanted in GR2 mice. The GR3 was treated with Cis (40 μg/mouse) on the first day post-inoculation. The fourth, fifth, and sixth treatment groups received Gin, AGNPS, and Gin/AGNPS (0.4 mg/mouse for 6 days). The seventh treated group was injected with Cis (40 μg/mice) and then administered with Gin/AGNPS for 6 days. All mice were slaughtered to examine the biochemical, histological and immunohistochemical alterations in the kidney. The findings demonstrated a substantial increase in the EAC group's overall body weight. However, in contrast to (GR2), groups that received Cis, AGNPS, Gin, Gin/AGNPS, and Cis/Gin/AGNPS displayed a small percentage increase in body weight. Furthermore, EAC produced renal impairment, as demonstrated by increase in serum urea and creatinine levels. While the treatment group with Gin/AGNPS and Cis/Gin/AGNPS improved and reduced these differences in renal function stress. Histological and immunohistochemical staining verified these results. Present results imply that loaded ginger nanoparticles may possess anti-oxidant, anti-cancer, and renal protective qualities in addition to inhibiting the damage that EAC causes to the kidney dysfunction.