This research assesses Fragaria ananassa leaf efficacy as an antibacterial agent against multidrug resistance (MDR) bacteria. The following cross-sectional research was performed from August 2018 to March 2019. About 120 bacterial isolates were isolated from 600 patients (276 males and 334 females) admitted to the Liver Institute Hospital (LIH) ICU. Antibiotic susceptibility was assessed using the VITEK 2 Compact ID/AST system. Among the biochemically described bacterial isolates, Acinetobacter baumannii was found to be multidrug-resistant, and its molecular identification was carried out using the 16S rDNA marker gene. Different Solvents, such as methanol, ethanol, acetone, and ethyl acetate were employed separately to extract the chemicals from the strawberry leaves. These extracts were tested for their antibacterial efficacies against the isolated Acinetobacter baumannii. The agar well diffusion results showed that the antibacterial activities were subsequently 25, 21, 12, and 26 mm for ethanol, methanol, ethyl acetate, and acetone. Sterilization at a high, moist temperature did not diminish the extract's antibacterial activity. The leaf extract was efficacious against Acinetobacter baumannii, with a minimum inhibitory concentration of 2 mg/mL and a minimum bactericidal concentration of 4 mg/mL. In conclusion, both cold and heat-treated strawberry leaf extracts are efficient as a conventional therapy against multidrug-resistant bacteria.