Staphylococcus aureus is a common bacterial pathogen that can cause
various infections, including biofilm-related infections that are difficult to
treat. In this study, we investigated the effects of different carbohydrates and
plant extracts on the biofilm formation of three standard strains of S. aureus
obtained from different sources. We found that the adherence and biofilm
formation of the strains were affected by their source, age, and culture
conditions. Additionally, we found that Nigella sativa extract and certain
monosaccharides, disaccharides, and polysaccharides were effective in
reducing biofilm formation, with some degree of strain-specificity.
Furthermore, we found that starch had a concentration-dependent effect on
the biofilm formation of S. aureus strains. Finally, we investigated the
acidogenicity of different carbohydrates in LB broth and found that they
were less acidogenic than the control group, but with no significant
differences between the strains. Our findings suggest that plant extracts and
certain carbohydrates may have the potential as natural alternatives for
preventing and treating biofilm-related infections caused by S. aureus.