These studies are devoted to develop novel analytical methods to assay levofloxacin and florfenicol in different matrices; dosage forms, milk products, meat, and biological fluids. Veterinary drugs are consumed on a large scale whether administered as food additives or added to drinking water. Besides, these pharmaceuticals are provided for animals as a prophylactic agent, treatment of different diseases and to enhance their growth. veterinary drug residues in food are a significant concern, and their detection, prevention, and management are crucial to ensure food safety and protect consumer health. Therefore, for public health concern, regulatory authorities have established guidelines to control the limit of veterinary residues in animals and their different food products. This current review involves various methods that have been published to determine different veterinary drugs, this includes, spectrophotometry, electrochemical methods and different chromatographic methods All suggested methods are fully validated under guidance of International Conference of Harmonization to be suitable for application of the developed methods for their intended purpose.