Background: Tobacco use has escalated into a major public health crisis, says the World Health Organization. Tobacco use is directly responsible for the deaths of more than 6 million people every year, out of an estimated 1.3 billion smokers globally. This research set out to compare the average intraocular pressure (IOP) of people who smoke now, have smoked in the past, and have never smoked to see whether there is any connection between the two. Methods: The participants in this observational cross-sectional research were divided into three (3) groups: The first group, G1, consists of 75 patients who do not smoke. The second group, G2, contains 75 patients who have smoked in the past year or more. The third group, G3, contains 75 patients who have smoked in the last year or more. The results showed that the non-smoker, past-smoker, and smoker groups were significantly different according to IOP. Patients with diabetes mellitus alone, hypertension (HTN) alone, diabetes mellitus plus hypertension (DM+HTN), and no comorbidities were significantly different from one another, according to IOP data. An increase in intraocular pressure was significantly associated with smoking, as we can see from the data.