Escherichia coli is the most frequent infecting organism in acute infection. So, knowledge about the frequency and distribution of urinary tract infection (UTI) is important to improve infection control measures. The aim of this research was to determine the prevalence of bacteria isolated from urinary tract infection (UTI) in patients and determination of the antibiotic susceptibility patterns of the Gram-negative bacteria. We evaluated 43 positive culture patients were collected over a period of 6 months starting from Jun, 2022 until December 2022 on patients attending various clinical in Egypt. The bacteria were isolated from different ages. Gram-negative bacteria were responsible for 69.8% of the isolates, while Gram-positive were responsible for 30.2%. Escherichia coli was the most prevalent pathogen. Regarding the susceptibility profile, results found Imipenem was the effective antibiotic against bacterial isolates. This study showed that the frequency of Escherichia coli increases the probability of urinary tract infection. Also, this survey indicates the emergence of antibiotic resistant infections in the studied clinics. So, there is a need to improve the effectiveness of integrated infection control programs to control and manage nosocomial infections caused by highly resistant organisms