Objectives: Assess the safety and effectiveness of treating acne scars using fractional CO2 laser resurfacing and then applying topical insulin. Background: When used together, fractional CO2 laser and topical insulin may significantly enhance the appearance of post-acne scars, on par with other well-established therapy methods. Sources of Information: For the purpose of treating acne scars after the fact, we searched and reviewed the Medline databases (Pub Med and Medscape) for research that compared the efficacy of employing fractional CO2 alone with that of fractional CO2 combined with topical insulin up to the year 2023. Study Selection: Each study was carefully reviewed by experts in the field to ensure its inclusion. Inclusion was contingent upon them meeting the following requirements: This work is written and published in English. Featured in journals that undergo a rigorous peer review process. 3. Give an explanation of the difference between utilising fractional CO2 alone and using it in conjunction with topical insulin for the treatment of acne scars. When extracting data, studies were discarded if they did not meet the inclusion criteria. Ethical permission, established eligibility criteria, sufficient information, and well-defined evaluation metrics were all variables in determining the study's quality. For our concerned research outcomes, data were independently extracted from all qualifying studies utilising a data collecting form. Results show that fractional CO2 laser and topical insulin significantly enhance the treatment of acne scars.