Background: Vitiligo is an epidermal depigmentation condition where melanocytes and melanin disappear, affecting one's appearance and self-esteem. Lymphocytes are only one of several cell types that have the polymorphic enzyme serum adenosine deaminase (ADA) on their surface. It has a crucial role in T lymphocyte development and function. The goal of this study is to examine the levels of adenosine deaminase in the serum of vitiligo patients and compare them to those of healthy individuals. Topics and Techniques: Sixty vitiligo patients and twenty age- and sex-matched healthy volunteers made up the patients' group. The Vitiligo Extent Tensity Index was used to quantify the level of skin discoloration caused by vitiligo. ELISA kits were used to determine the total ADA (tADA) in the serum. There was no statistically significant difference in age or gender between the patients and the control group. The median levels of tADA were drastically different between the patient and control groups. It is likely that serum tADA contributes to the aetiology of vitiligo. New therapy options for vitiligo patients may benefit from the information that this research may give.