Background: Ovarian failure, characterized by the loss of ovarian function, presents a significant challenge for women seeking to conceive. Conventional treatments have limitations in restoring ovarian function and fertility.. The aim of this study was to assess the influence of intraovarian injection of PRP on ovarian function in a case with ovarian failure. Methods: This prospective interventional multi-center study included 50 women with primary and secondary ovarian failure selected from Obstetrics Department, Benha University Hospitals & other private centers. All patients were subjected to complete history taking, clinical examination, laboratory investigations including hormonal Laboratory evaluation of (FSH, LH, estradiol, AMH), PRP injection under intravenous anesthesia and post-operative follow up mainly for measuring ovarian hormones: FSH, LH, AMH, E2 every month for 6 months. Results: The current study included 50 patients. The mean age of them was (36.68 ± 6.36 years); range from (24 –45years). The median age (Q1, Q3) was 38 (32.75, 42). The mean serum follicle stimulating hormone (FSH) was 38.82 ± 20.38 (range 7.03 to 90.2) and its median (Q1, Q2) was 36.75 (19.75, 57). The mean serum luteinizing hormone (LH) was 21.43 ± 14.36 (range 4.3 to 65.5) and its median (Q1, Q2) was 16.9 (11.3, 28.7). The mean serum Estradiol hormone (E2) was 29.87 ± 22.72 (range 5 to 96) and its median (Q1, Q2) was 22.16 (13.1, 38.25). Conclusions: In the study group, intraovarian injection of PRP decreased FSH, LH, and increased E2 levels significantly, however it was less effective for boosting AMH levels.