developing a high-performance liquid chromatography separation technique that is quick and precise and quantification of both spectinomycin sulphate tetrahydrate and lincomycin hydrochloride in the veterinary formulation drug. This method is applied on an HPLC Agilent 1260 with a UV detector using 150X 4.6 mm BDS hypersil cyano column, Particle size 5 µm. Mobile phase: 95% buffer (dissolve 3.5 g potassium dihydrogen phosphate in 1000 ml distilled water and adjust pH to 6.5 with 1N sodium hydroxide):5 % acetonitrile, Flow rate 1.0 ml/min, Wavelength 220 nm, Injection volume 10.0 µl and ambient column temperature.. The calibration curve of spectinomycin sulphate tetrahydrate and lincomycin hydrochloride was linear with a correlation coefficient that should be more than 0.99 between 50% and 150% of the desired concentrations. It is determined by preparing five concentrations of spectinomycin sulfate tetrahydrate (6.72, 10.08, 13.44, 16.8 and 20.16 mg/ml) and lincomycin hydrochloride (2.513, 3.7695, 5.026, 6.2825 and 7.539 mg/ml). Linearity results of spectinomycin sulfate r=0.9999 NLT, Linearity results for Lincomycin hydrochloride = 0.999 so the method is found to be linear as r is more than 0.99, The result of relative standard deviation was ≤2. The suggested technique was verified and successfully used for the separation and quantification of both spectinomycin sulphate and lincomycin hydrochloride in the veterinary drug.

developing a high-performance liquid chromatography separation technique that is quick and precise and quantification of both spectinomycin sulphate tetrahydrate and lincomycin hydrochloride in the veterinary formulation drug. This method is applied on an HPLC Agilent 1260 with a UV detector using 150X 4.6 mm BDS hypersil cyano column, Particle size 5 µm. Mobile phase: 95% buffer (dissolve 3.5 g potassium dihydrogen phosphate in 1000 ml distilled water and adjust pH to 6.5 with 1N sodium hydroxide):5 % acetonitrile, Flow rate 1.0 ml/min, Wavelength 220 nm, Injection volume 10.0 µl and ambient column temperature.. The calibration curve of spectinomycin sulphate tetrahydrate and lincomycin hydrochloride was linear with a correlation coefficient that should be more than 0.99 between 50% and 150% of the desired concentrations. It is determined by preparing five concentrations of spectinomycin sulfate tetrahydrate (6.72, 10.08, 13.44, 16.8 and 20.16 mg/ml) and lincomycin hydrochloride (2.513, 3.7695, 5.026, 6.2825 and 7.539 mg/ml). Linearity results of spectinomycin sulfate r=0.9999 NLT, Linearity results for Lincomycin hydrochloride = 0.999 so the method is found to be linear as r is more than 0.99, The result of relative standard deviation was ≤2. The suggested technique was verified and successfully used for the separation and quantification of both spectinomycin sulphate and lincomycin hydrochloride in the veterinary drug.