Background: Perimenopause refers to the time period around menopause, during which the body makes the transition to menopause. Endometrial cancer is the sixth most prevalent cancer among women globally and the fifteenth most prevalent cancer overall. Aim of the research: assessing the instructional guidelines effect on knowledge and attitudes of perimenopausal women regarding endometrial cancer. Research design: A quasi-experimental design. Research setting: The current research were carried out at faculty of nursing and faculty of education, Benha University. Sample type: A convenient sample was selected from the above-mentioned research setting. Sample size: All administrated women at the Faculty of Nursing (30) and Faculty of Education (55) Benha University > 42 years during the time of data collection, (Total sample size 85). Tools of data collection: two main tools were utilized: tool (I) Self administrated questionnaire sheet, it covered: Socio-demographic characteristics such as (age, social status, education level, residence and family income), History of studied women such as (birth control history, menstrual and obstetric history, medical history and surgical history) and assessment women's knowledge regarding endometrial cancer, tool (II) Modified likert scale for menopausal women's attitudes regarding endometrial cancer. Result: There was a significant positive correlation between women's total knowledge and their attitudes regarding endometrial cancer before and after instructional guidelines implementation (p ≤ 0.001). Conclusion: Instructional guidelines implantation had a positive effect on women's attitudes and knowledge regarding endometrial cancer. Recommendation: To avoid endometrial cancer, implement a countrywide screening program for women to detect any alterations in the reproductive system.