Background: Wound healing is a set of elaborate and complex mechanisms including period of oscillation of inflammation, granulation tissue production, and tissue remodeling, which culminates in the restoration of tissue structural stability and injury. That study purposed to evaluate clinical and histopathological changes of keloid and hypertrophic scars before and after CO₂ laser treatment. Methods: That interventional research was carried out on individuals with keloids and hypertrophic scars attending the Out-patient Clinic of Dermatology in Hospital of Benha University. Participants were exposed to historical analysis, Local Dermatological inspection, Tissue biopsy, Treatment course by fractional CO₂ laser, follow up after treatment. Results: A statistically critical decrease in size of the lesion when comparing among pretreatment and post-treatment [p < 0.05]. Vancouver scale score results were considerably higher pre-treatment than post-treatment [P<0.05]. Conclusion:From the previous results we can conclude that Fractional CO2 laser is a viable therapy option for keloid and hypertrophic scars since it reduces clinical signs such as colour, thickness, and pruritus in addition to improvement at the cellular level.