Bacterial resistance to antibiotics drugs in a continuous development due to the wrong and excessive use of antibiotics, a malty-drug resistant bacteria became a real danger so there is a necessity to search for alternatives strategies to improve the quality of antibiotics or find alternative materials, in recent years, numerous studies confirm various bioactivities of natural products among the antibacterial activity. The aim of this study was to investigate the ability of homogenous whole bee extract of Apis mellifera to overcome microbial resistance to antibiotics as natural antimicrobial agent besides its activity as a complementary to increase the quality, potency and the ability of the antibiotic against resistance bacteria has been investigated. Whole bee extract has been tested on multi drug resistant bacteria isolates from different kind of human infections separately as antimicrobial agent alone beside using by mixing with the tested antibiotics agents that  have been used in the study.  The results of this study indicate a significant effect of antimicrobial activity by homogenous whole bee extract against the isolate Pseudomonas aeruginosa and Klebsiella pneumonia then which bacterial growth inhibition observed in isolates beside the significant antimicrobial effects of the bee extract on the bacterial isolates some complementary effects with the resistance antibiotics in which homogenous bee extract indicate complementary effect for most types of tested antibiotics been observed in that case bee extract could have antimicrobial effects on resistant bacteria to antibiotics and improving the quality and ability of the resistance antibiotics to be more significant.