Type 1 diabetes is regarded as a chronic autoimmune disease characterized by dysfunctional T-lymphocyte activation that targets pancreatic Langerhans β-cells, causing insulin deficiency. By raising the amounts of sugar in the blood and urine, this condition causes hyperglycemia. Five groups were chosen for the current experiment, with N standing in for healthy individuals. Four groups of patients were created based on their ages. The bidirectional sequence of the HLA-DRB1 exon 2 gene was determined using blood samples from the five groups. PCR was used to define the gene sequence, amplify it, and isolate the gene for DNA sequencing in two directions. To understand more about the isolated gene and its role in T1DM, bioinformatics analysis of the sequencing output data was carried out. In addition, certain software programs were used by the study teams to forecast the translated protein and RNA secondary structure. The findings showed that an alteration in the secondary structure of RNA affected protein translation and release in bodily cells. Additionally, based on SNP analysis, the four groups' targeted genes revealed mutations caused by the replacement or indel of certain nucleotides, which change the anticipated translated protein in patient groups in comparison to control.