Aim: The objective of this retrospective study was to assess the prevalence of the mandibular incisive canal (MIC) and evaluate the morphometric parameters through the utilization of cone beam computed tomography (CBCT). Materials and methods: Two hundred and sixty-four CBCT scans of patients aged 18 to 70 were part of this study, including both genders. After confirming the presence of MIC, its length was determined, and subsequent measurements were taken based on its extension in the canine, lateral incisor, and first premolar regions. These measurements included the lingual bony surface, the distance between the labial bony surface and the canal, inferior border, and alveolar bone crest. Results: The prevalence of MIC was observed in 121 cases (45.8%), with an average length of 9.91 mm. The distance from the canal to the alveolar crest at the first premolar, canine, and lateral incisors were 18.44 mm, 18.99 mm, and 16.14 mm, respectively. Moreover, the distances to the inferior border were 8.79 mm, 9.39 mm, and 9.21 mm, respectively; to the labial bone surface were 3.05 mm, 3.35 mm, and 3.14 mm, respectively; and to the lingual bone surface were 4.83 mm, 4.64 mm, and 5.94 mm, respectively. Conclusion: Greater attention is warranted from radiologists and surgeons to avoid mandibular incisive nerve damage during different dental treatments. Therefore, using CBCT to identify the existence and location of the MIC is crucial.