Aim: This study was based on evaluating the bactericidal ability of diode laser (980 nm) in root canals of primary teeth infected with Enterococcus faecalis. Materials and methods: Forty-eight primary molar teeth were collected and divided into four groups (n = 12). Group I: Chlorhexidine (CHX), Group II: 980 nm diode laser, Group III: CHX + diode laser, Group IV: Saline (positive control). Distal canals were separated, cleaned and shaped using manual K-files, sterilized then infected with E.feacalis. Samples from the infected root canals were obtained and cultured on agar. The numbers of CFU were counted after each intervention at 24 h and at 7 days. Data were analyzed by Post hoc pairwise comparisons. Results: The highest percentage of bacterial reduction in 24 h and 7 days was found in laser/CHX group and CHX group with no significant difference between these two groups at both time intervals (p>0.05). Followed by laser group while the saline group had the lowest value after 24 h and 7 days Conclusion: Laser can be used as successful approach solely or in conjunction with biocompatible irrigating materials in pediatric patients for disinfecting root canal