Abstract:
Aim: The aim of the current study was to assess the effect of thymoquinone (TQ) on tongue squamous cell carcinoma cell line (HNO-97).
Materials and Methods: Tongue squamous carcinoma cell line (HNO-97) was obtained, and maintained in cultured flasks and were divided into; Group Ι (Control): Untreated (HNO-97) cells and three treated groups: HNO-97-treated cells with three different concentrations of TQ. All cells were incubated for 24 h. The viability of HNO-97 cells treated with TQ was determined 24 h post treatment. Determination of the effect of TQ on HNO-97 cell line was done by Methyl Thiazol Tetrazolium (MTT) Cytotoxicity Assay, flow cytometry cell cycle assay, and ELISA test by detection of TNF-α.
Results: MTT assay showed that TQ cytotoxicity was in dose dependent. The mean percentage of viability of the treated cells decreased in a dose dependent manner. Moreover, TQ treatment induced reduction in the percentage of cells in the G1 to S phases of the cell cycle and increased the percentage of cancer cells in the G2/M phase. Furthermore, TQ treatment significantly decreased TNF-α level in a dose dependent manner.
Conclusion: The effect of TQ decreased the viability of Tongue Squamous Cell Carcinoma Cell Line in a dose dependent manner.