Aim: With the help of mediating agents such as plants and microbes, silver nanoparticles can be functionalized with molecular capping agents allowing a wide range of applications in the field of dentistry especially in terms of their antimicrobial efficacy. Thus the present study was designed with an aim to synthesise silver nanoparticles mediated by Phyllanthus emblica and Vaccinium oxycoccos fruit extract that contain bioactive metabolites and to study its characteristics and antimicrobial efficacy.
Materials and Method: The green approach of synthesizing silver nanoparticles involved the use of fruit extracts from Phyllanthus emblica & Vaccinium oxycoccos. The produced nanoparticles were characterized by means of SEM (Scanning Electron Microscopy), EDAX (Energy-Dispersive X-ray) analysis, and FTIR (Fourier Transform Infrared) spectroscopy. To assess the synthetic nanoparticles' antibacterial activity against particular strains of Staphylococcus aureus, Streptococcus mutans, & Enterococcus faecalis, the Agar well diffusion method was employed.
Results: The fruit extract & the new nanoparticle both showed the presence of different functional groups. The SEM study showed that the particles were spherical, & the EDX investigation verified the existence of the silver element. The nanoparticle showed a dose-dependent antimicrobial efficacy which was highest at 100μL for all the three microorganisms.
Conclusion: Instead of using harmful chemicals to synthesize silver nanoparticles, fruit extract from Phyllanthus emblica and Vaccinium oxycoccos can be used because of its quantity of naturally occurring bioactive compounds and effectiveness against different microbial strains.