The current investigation was conducted to investigate the relationship between ingestion of aflatoxin already formed in ration and level of aflatoxin M1 secreted in milk. A total of 300 samples were collected from animal ration, cow, and buffalo milk samples (100 samples for each from the same farm) from different dairy farms in El Menofia Governorate. The Mean mould count in the examined ration samples, cow milk samples and buffalo milk samples were 4.03 ± 0.40,3.34± 0.31and2.85 ± 0.26log cfu ̸ ml, respectively. Identification of mould isolates in ration samples, cow milk samples and buffalo milk samples were Aspergillus niger isolated in a percentage of  15, 30 and 28, respectively, while Aspergillus flavus isolated in a percentage of  20, 12 and 14, respectively. On the other hand, the number of Aspergillus fumigatus isolated in a percentage of 8, 8 and 12 of examined samples, respectively. The number of Penicillium speciesof the same samples were 4, 25 and 18, respectively. Fusarium species were isolated in a percentage of 25, 20 and 15, respectively. The number of Mucor species isolated in a percentage of 28, 5 and 13, respectively .The mean level of aflatoxin in 57 ration samples was 29.578±0.80 (PPb) ̸ kg. while the mean level of aflatoxin M1 was 0.079±0.003ppb ̸ kg and 0.068±0.002ppb/ kg. In cow and buffalo milk samples respectively. In conclusion, The levels of contaminated animal^''s feed and milk obtained in this study with aflatoxin (AF) and AFM1 should serve as a cautionary call for strict control of raw materials and feed samples to prevent exposure of livestock to aflatoxin contaminated feed which could result in the release of AFM1 into milk and ultimately cause human endangerment through consumption of contaminated milk.