Functionalized multiwalled carbon nanotubes (FMWNTs) were used in this study to improve the performance of polyvinyl (PVA). The hydrogen-bonding interactions between polyvinyl alcohol and nanofillers provided the driving force for the creation of the homogenous dispersion nanocomposite films between the FMWCNTs into the polymer matrix. Antimicrobial activity, tensile strength tan δ, transmission electron microscopy, thermal gravimetric analysis and Fourier transform infrared were among the techniques used to examine the biological, mechanical and structural performance of the prepared nanocomposite.  Because of their homogeneous dispersion across the PVA matrix, the nanofillers considerably improved the film's mechanical and biological activity.