A total of 60 samples (30 poultry feed and 30 chicken liver samples) were collected randomly from some farms and localities in Beni-Suef Governorate in summer and winter seasons for monitoring mycotoxin contamination condition in such farms. Samples were examined for aflatoxin and ochratoxin levels. All samples (poultry feed and chicken liver samples) were found contaminated with both mycotoxins. Concerning , poultry feed samples in summer, aflatoxin levels ranged from 1.10 to 65 ppb with mean of 8.79±4.11, one sample (6.66%) exceeded the permissible limit (20 ppb); While, ochratoxin levels ranged from 3.50 to 9.90 ppb with mean of 5.51±0.52, eight samples (53.33%) exceeded the permissible limit (5 ppb). In winter, aflatoxin levels ranged from 0.15 to 7.70 ppb with mean of 3.01±0.93, all samples were within the permissible limit (20 ppb); while, ochratoxin levels ranged from 2.50 to 11.90 ppb with mean of 4.55±0.70, four samples (26.66%) exceeded the permissible limit (5 ppb). Chicken liver samples were found contaminated with aflatoxin levels ranged from 0.51 to 6.52 ppb with mean of 2.15±0.72, all these levels were within FDA permissible limit (20 ppb); nine samples (30%) exceeded FAO permissible limits (4 ppb). While, ochratoxin levels ranged from 1.90 to 5.40 ppb with mean of 2.55±0.32, three samples (10%) exceeded FAO permissible limits (5 ppb); all samples (100%) exceeded Italian permissible limits (1 ppb).
Such result was the motive to carry out a study aimed to reduce the toxicity of aflatoxin and ochratoxin in broiler chickens by using of Hydrated Sodium Calcium Aluminosilicate (HSCAS). Fifty, one-day-old broiler chicks were divided equally into five groups. Birds of group (1) kept as control, group (2) fed aflatoxin 100 ppb in ration, group (3) fed aflatoxin 100 ppb plus 1% HSCAS in ration, group (4) fed ochratoxin  50 ppb in ration and group (5) fed ochratoxin 50 ppb plus 1% HSCAS in ration. All groups were kept under close observation until 35 days of age; clinical sings, mortalities and body weight were recorded. At the end of the experiment, muscles and liver samples were obtained from all groups for mycotoxins estimation by using LC_MS/MS. Mycotoxins negatively affected the body weight of birds while, mortalities were increased. Addition of 1% HSCAS induced significant improvement on body weight reduction and mortalities. Mycotoxins residues were found in liver and muscles of birds fed contaminated rations, birds fed rations treated with HSCAS showed lower levels than those untreated. We concluded that HSCAS can be a useful approach to reduce the toxicity of aflatoxin and ochratoxin in broilers.