C
ryptosporidiosis is one of the most important causes of neonatal enteritis and diarrhea in calves leading to significant morbidity and mortality rates globally. This study was conducted to highlight the diagnostic methods of cryptosporidiosis in pre-weaned cattle calves. For this purpose, a total of 88 diarrheic faecal samples of pre-waned calves at Giza and El-Fayoum governorates, Egypt, were microscopically examined using Modified Ziehl-Neelsen staining (MZN), Immunochromatographic test (ICT) and Enzyme linked Immunosorbent Assay (ELISA). The prevalence of infection was 71.59% (63/88), 73.86% (65/88), and 75.00% (66/88) for MZN, ICT, and ELISA respectively. The Sensitivity was 96.9%, 100%, and 100% whereas; Specificity was 100%, 100%, and 95.7% for MZN, ICT, and ELISA, respectively