A total of 69 cloacal swabs were collected from turkey from different turkey farms in Giza and Sharkia governorates in Egypt for isolation of Campylobacter species.The results revealed that Campylobacter was isolated from 16 out of 69 (23.1%) cloacal samples. The samples were identified biochemically revealed that 4 out of 16 (25%) were identified as Campylobacter jejuni (C. jejuni) while 12 out of 16 (75%) were Campylobacter coli (C. coli).  Campylobacter isolates were confirmed by multiplex PCR using hipO gene which responsible for Na hippurate hydrolysis for confirmation of C. jejuni and ceuE gene which designed for detection of C. coli. All isolates were examined for presence of tetracycline resistance gene tetM was detected in 8 out of 16 (50%) Campylobacter isolates. The cdtB virulence gene was detected in all isolates. From mentioned results we recommended the use of multiplex polymerase chain reaction (PCR) for identification of Campylobacter species. This study may indicate the extent of the existence of campylobacter species in some turkey farms in Giza &Sharkia governorates. In conclusion, biosecurity  programs must be applied inside slaughter houses to avoid carcass contamination. Applying more efforts in surveillance programs in turkey farms for epidemiological mapping of Campylobacter existence and antimicrobial resistance distribution which obligate the stop of uncontrollable use of antibiotics in poultry farms.