Avian infectious bronchitis virus (IBV) is still the cause of huge economic losses among poultry farms despite extensive vaccination. During this study, clinical samples were collected from four Egyptian governorates in the period 2021-2022. Samples with positive IBV real-time RT-PCR results were subjected to virus isolation on chicken embryos. IBV was identified by RT-PCR that was directed to the S1 gene. Partial sequencing of S1 gene revealed seven strains (HS1-7) that were submitted to GenBank under the accession numbers (OQ349378-OQ349384). Phylogenetic analysis revealed clustering of five strains (HS1-5) with genotype I- lineage 23(GI-23), whereas the other two strains (HS6 and HS7) were clustered with genotype I- lineage 1 (GI-1). Strains HS1-3 were found to have a unique amino acid insertion in hypervariable region 1 (HVR1). GI-23-related (variant) strains shared less than 86.2% and 88.4% nucleotide and amino acid identities, respectively with the commonly used vaccine strains. The closest vaccine strain (EG/1212B) showed 9-15 amino acid substitutions within HVR1 and 2. Although GI-1-related strains shared close relation to Mass like vaccine strains, 5-13 amino acid substitutions were observed within HVR1 and 2. This study reports the identification of genetically distinct IBV strains in different Egyptian governorates with probable impact on the protection attracted by commercial vaccines.