A Field study was conducted at EL-Beheira Governorate, Egypt to determine the insecticidal activities of pyridalyl, methomyl, emamectin benzoate, and lufenuron on tomato plants against the cotton leaf-worm (Spodotera Littoralis). Data obtained showed that a pyridalyl compound has the highest toxicity against the cotton leaf worm followed by methomyl then emamectin benzoate while the lufenuron has the lowest toxicity. The insecticide residue on the fruits of treated tomato fruits was extracted using QuEChERS method before analysis by HPLC-UV. The validation method for extraction and quantitative analysis of tested pesticides residue in tomato fruits using HPLC-UV, at fortification levels of 0.1, 0.5, and 1.0 mg/kg in tomato fruits was performed. The results suggest that the pyridalyl, methomyl, emamectin benzoate, and lufenuorn dissipation curves and its half-lives in tomatoes were 1.16, 1.38, 1.80 and 1.20 days for pyridalyl, methomyl, emamectin benzoate, and lufenourn, respectively. The residues in tomato were below the (MRL) and the safety time was 1, 5, 7 & 7 days for pyridalyl, methomyl, emamectin benzoate, and lufenuron, respectively. In conclusion, pyridalyl, methomyl, emamectin benzoate, and lufenuron insecticide are useful in controlling cotton leaf-worms in tomato fields and safe for human consumption.