This study focused on identifying the preventive effect of prunes & raisins mixture and muffin stuffed with them on rats injured with irritable bowel syndrome. Rats male albino weighing 170 ± 15 g (n=30), were randomly assigned to five groups of 6 rats per each.

The 1st group fed basal diet was served as a normal control(-ve), and the remaining four groups were classified into control positive (+ve) group (fed on basal diet only), groups of 3, 4, and 5 were fed basal diet containing 20% of (normal muffins (N), or best muffin formula or dried fruit mixture). Irritable bowel syndrome was induced after 29 days from the beginning of experiment by using 50 µl mustard oil (1% in 70% ethanol) by intra colonic administration.
The results indicated that the best muffin formula recorded the highest total phenols content (427.60 mg/100g), and total antioxidant activity (45.44%) comparing to normal muffin. Irritable bowel syndrome rats group fed the best muffin formula showed significant increase in some nutritional parameters as weight gain, food intake and food efficiency ratio comparing with (+ve) positive control group, decreased levels of free radicals (malondialdehyde (MDA) and hydrogen peroxide (H2O2), while increasing in antioxidant enzymes such as, glutathione peroxidase (GSH), superoxide dismutase (SOD) and catalase activity (CAT).