Herbal remedies are now associated with safety, in contrast to synthetics, which are seen to be harmful to persons and the environment. This investigation aimed to test in vivo the effectiveness of herbs oils which were chosen for their polyphenol content, had antimicrobial activity on oral bacteria. The antibacterial activity of myrrh (Commiphora molmol), thyme (Thymus vulgaris L.), sage (Salvia officinalis L.), chamomile (Matricaria recutita L.) and green tea (Camellia sinensis) essential oils (5 mg oil/100 ml water) dilution against total bacterial counts in saliva was studied. The counts of Streptococcus mutans and Lactobacillus rhamnosus were investigated. The minimum inhibitory zones (MIZ) of myrrh, thyme, sage, chamomile and green tea essential oils on Streptococcus mutans and Lactobacillus rhamnosus were determined using a standard susceptibility agar dilution technique to investigate their potential use as antibacterial agents. Data were statistically processed. The experimental group showed a statistically significant reduction in colony counts of Streptococcus mutans and Lactobacillus rhamnosus relative to the control group. These findings showed the efficacy of the tested herbs oils against halitosis and oral flora, achieving a balance between the harmful and the harmless oral bacteria and also opening a promising avenue of clinical applications in the preparation of specific and natural antibacterial remedies.