Background: These days, using a computer or other visual display device is almost a need. Consequently, a great deal of people worldwide experiences a range of visual symptoms such as; dry eyes, ocular strain, ocular irritation, and ocular redness. Computer vision syndrome (CVS) is the term used to describe all of these visual abnormalities. Aim of the study: This study aimed to evaluate the effect of structured teaching guidelines on nursing students' awareness regarding computer vision syndrome. Study design: Quasi-experimental pre/ post research design was used to conduct this study. Setting: This study was conducted in the Faculty of Nursing, Benha University, Qalyubia Governorate, Egypt. Study subjects: Convenience sample of the second year students (303) from both sexes in the previously mentioned setting during the time of data collection and agreed to participate in this study. Tools of data collection: Two tools were used; Tool (1) Structured interview questionnaire involving three parts: Part one: student's personal data, Part two: students' knowledge regarding computer vision syndrome and Part three: Presence of symptoms after using computer. Tool (2) Awareness level scale about CVS. Results: Regarding students' total knowledge about CVS pre and post teaching guidelines implementation, none of the students (0.0%) had good knowledge about computer specific preventive measures pre-teaching guidelines implementation. While it improved as (64.0%) had good knowledge post-teaching guidelines implementation. Nursing students' awareness mean score about CVS improved from 33.26 ± 10.31 pre-guidelines implementation to 36.81± 11.23 post-guidelines implementation. Conclusion: Teaching guidelines has proven to be highly effective in improving nursing students' awareness regarding CVS, which supports the research hypothesis. Recommendations: Developing an ergonomic training program as part of the initial training program of all computer users.